6MH2

Structure of Herceptin Fab without antigen

Summary for 6MH2

• Entry DOI: 10.2210/pdb6mh2/pdb
• Descriptor: Herceptin Fab arm light chain, Herceptin Fab arm heavy chain (2 entities in total)
• Functional Keywords: herceptin, antibody, fab fragment, immune system
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 4
• Total formula weight: 96367.25

Authors

Luthra, A.,Langley, D.B.,Christie, M.,Christ, D. (deposition date: 2018-09-17, release date: 2019-05-29, Last modification date: 2024-10-23)

Primary citation

Luthra, A.,Langley, D.B.,Schofield, P.,Jackson, J.,Abdelatti, M.,Rouet, R.,Nevoltris, D.,Mazigi, O.,Crossett, B.,Christie, M.,Christ, D.
Human Antibody Bispecifics through Phage Display Selection.
Biochemistry, 58:1701-1704, 2019

PubMed Abstract: We developed a repertoire approach to generate human antibody bispecifics. Using phage display selection of antibody heavy chains in the presence of a competitor light chain and providing a cognate light chain with an affinity handle, we identified mutations that prevent heavy/light chain mispairing. The strategy allows for the selection of human antibody chains that autonomously assemble into bispecifics.

PubMed: 30900875
DOI: 10.1021/acs.biochem.9b00037

Experimental method

X-RAY DIFFRACTION (2.8 Å)