6MCB

CryoEM structure of AcrIIA2 in complex with CRISPR-Cas9

Summary for 6MCB

Entry DOI	10.2210/pdb6mcb/pdb
EMDB information	9066 9067
Descriptor	Single guide RNA (116-MER), CRISPR-associated endonuclease Cas9, Anti-CRISPR protein AcrIIA2 (3 entities in total)
Functional Keywords	crispr-cas, cas9, cas9 inhibitors, anti-crispr, acriia2, bacteriophage, gene editing, hydrolase-rna-viral protein complex, hydrolase/rna/viral protein
Biological source	Streptococcus pyogenes M1 GAS More
Total number of polymer chains	3
Total formula weight	210495.68
Authors	Jiang, F.,Liu, J.J.,Doudna, J.A. (deposition date: 2018-08-31, release date: 2019-01-16, Last modification date: 2024-03-13)
Primary citation	Jiang, F.,Liu, J.J.,Osuna, B.A.,Xu, M.,Berry, J.D.,Rauch, B.J.,Nogales, E.,Bondy-Denomy, J.,Doudna, J.A. Temperature-Responsive Competitive Inhibition of CRISPR-Cas9. Mol. Cell, 73:601-, 2019 Cited by PubMed: 30595438 DOI: 10.1016/j.molcel.2018.11.016 PDB entries with the same primary citation
Experimental method	ELECTRON MICROSCOPY (3.4 Å)

Structure validation