6M6O

NMR SOLUTION STRUCTURE OF A C-FLIPs

6M6O の概要

• エントリーDOI: 10.2210/pdb6m6o/pdb
• 分子名称: CASP8 and FADD-like apoptosis regulator (1 entity in total)
• 機能のキーワード: apoptosis, c-flips
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21588.22

構造登録者

Bai, Z.Q.,Hu, K.F. (登録日: 2020-03-16, 公開日: 2021-03-17, 最終更新日: 2024-05-15)

主引用文献

Bai, Z.Q.,Ma, X.,Liu, B.,Huang, T.,Hu, K.
Solution structure of c-FLIP death effector domains.
Biochem.Biophys.Res.Commun., 617:1-6, 2022

PubMed Abstract: The formation of death-inducing signaling complex (DISC) and death effector domain (DED) filament initiates extrinsic apoptosis. Recruitment and activation of procaspase-8 at the DISC are regulated by c-FLIP. The interaction between c-FLIP and procaspase-8 is mediated by their tandem DEDs (tDED). However, the structure of c-FLIP and how c-FLIP interferes with procaspase-8 activation at the DISC remain elusive. Here, we solved the monomeric structure of c-FLIP (F114G) at near physiological pH by solution nuclear magnetic resonance (NMR). Structural superimposition reveals c-FLIP (F114G) adopts a structural topology similar to that of procaspase-8. Our results provide a structural basis for understanding how c-FLIP interacts with procaspase-8 and the molecular mechanisms of c-FLIP in regulating cell death.

PubMed: 35688044
DOI: 10.1016/j.bbrc.2022.05.086

実験手法

SOLUTION NMR