6M3C

hAPC-h1573 Fab complex

6M3C の概要

• エントリーDOI: 10.2210/pdb6m3c/pdb
• 分子名称: Vitamin K-dependent protein C heavy chain, Vitamin K-dependent protein C light chain, h1573 Fab H chain, ... (4 entities in total)
• 機能のキーワード: human apc, h1573 fab, complex, blood clotting, blood clotting-immune system complex, blood clotting/immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 279683.55

構造登録者

Wang, X.,Wang, D.,Zhao, X.,Egner, U. (登録日: 2020-03-03, 公開日: 2020-07-08, 最終更新日: 2024-10-16)

主引用文献

Zhao, X.Y.,Wilmen, A.,Wang, D.,Wang, X.,Bauzon, M.,Kim, J.Y.,Linden, L.,Li, L.,Egner, U.,Marquardt, T.,Moosmayer, D.,Tebbe, J.,Gluck, J.M.,Ellinger, P.,McLean, K.,Yuan, S.,Yegneswaran, S.,Jiang, X.,Evans, V.,Gu, J.M.,Schneider, D.,Zhu, Y.,Xu, Y.,Mallari, C.,Hesslein, A.,Wang, Y.,Schmidt, N.,Gutberlet, K.,Ruehl-Fehlert, C.,Freyberger, A.,Hermiston, T.,Patel, C.,Sim, D.,Mosnier, L.O.,Laux, V.
Targeted inhibition of activated protein C by a non-active-site inhibitory antibody to treat hemophilia.
Nat Commun, 11:2992-2992, 2020

PubMed Abstract: Activated protein C (APC) is a plasma serine protease with antithrombotic and cytoprotective functions. Based on the hypothesis that specific inhibition of APC's anticoagulant but not its cytoprotective activity can be beneficial for hemophilia therapy, 2 types of inhibitory monoclonal antibodies (mAbs) are tested: A type I active-site binding mAb and a type II mAb binding to an exosite on APC (required for anticoagulant activity) as shown by X-ray crystallography. Both mAbs increase thrombin generation and promote plasma clotting. Type I blocks all APC activities, whereas type II preserves APC's cytoprotective function. In normal monkeys, type I causes many adverse effects including animal death. In contrast, type II is well-tolerated in normal monkeys and shows both acute and prophylactic dose-dependent efficacy in hemophilic monkeys. Our data show that the type II mAb can specifically inhibit APC's anticoagulant function without compromising its cytoprotective function and offers superior therapeutic opportunities for hemophilia.

PubMed: 32532974
DOI: 10.1038/s41467-020-16720-9

実験手法

X-RAY DIFFRACTION (3.7 Å)