6M33

Crystal structure of Homo Sapian GCP6 N-terminus and Mozart1

6M33 の概要

• エントリーDOI: 10.2210/pdb6m33/pdb
• 分子名称: Gamma-tubulin complex component 6, Mitotic-spindle organizing protein 1 (2 entities in total)
• 機能のキーワード: gamma tubulin complex, microtubule, gcp, mzt1, cell cycle
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22353.85

構造登録者

Huang, T.L.,Hsia, K.C. (登録日: 2020-03-02, 公開日: 2021-02-03, 最終更新日: 2024-03-27)

主引用文献

Wieczorek, M.,Huang, T.L.,Urnavicius, L.,Hsia, K.C.,Kapoor, T.M.
MZT Proteins Form Multi-Faceted Structural Modules in the gamma-Tubulin Ring Complex.
Cell Rep, 31:107791-107791, 2020

PubMed Abstract: Microtubule organization depends on the γ-tubulin ring complex (γ-TuRC), a ∼2.3-MDa nucleation factor comprising an asymmetric assembly of γ-tubulin and GCP2-GCP6. However, it is currently unclear how the γ-TuRC-associated microproteins MZT1 and MZT2 contribute to the structure and regulation of the holocomplex. Here, we report cryo-EM structures of MZT1 and MZT2 in the context of the native human γ-TuRC. MZT1 forms two subcomplexes with the N-terminal α-helical domains of GCP3 or GCP6 (GCP-NHDs) within the γ-TuRC "lumenal bridge." We determine the X-ray structure of recombinant MZT1/GCP6-NHD and find it is similar to that within the native γ-TuRC. We identify two additional MZT/GCP-NHD-like subcomplexes, one of which is located on the outer face of the γ-TuRC and comprises MZT2 and GCP2-NHD in complex with a centrosomin motif 1 (CM1)-containing peptide. Our data reveal how MZT1 and MZT2 establish multi-faceted, structurally mimetic "modules" that can expand structural and regulatory interfaces in the γ-TuRC.

PubMed: 32610146
DOI: 10.1016/j.celrep.2020.107791

実験手法

X-RAY DIFFRACTION (3.29434890409 Å)