6M0P

Hydroxylamine oxidoreductase in complex with juglone

6M0P の概要

• エントリーDOI: 10.2210/pdb6m0p/pdb
• 分子名称: Aerobic hydroxylamine oxidoreductase, Uncharacterized protein, HEME C, ... (8 entities in total)
• 機能のキーワード: enhancer, complex, oxidoreductase
• 由来する生物種: Nitrosomonas europaea; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 238113.02

構造登録者

Fujiwara, T.,Fujimoto, Z.,Nishigaya, Y.,Yamazaki, T. (登録日: 2020-02-22, 公開日: 2021-03-10, 最終更新日: 2025-09-17)

主引用文献

Akutsu, Y.,Fujiwara, T.,Suzuki, R.,Nishigaya, Y.,Yamazaki, T.
Juglone, a plant-derived 1,4-naphthoquinone, binds to hydroxylamine oxidoreductase and inhibits the electron transfer to cytochrome c 554.
Appl.Environ.Microbiol., 89:e0129123-e0129123, 2023

PubMed Abstract: Nitrification, the microbial conversion of ammonia to nitrate via nitrite, plays a pivotal role in the global nitrogen cycle. However, the excessive use of ammonium-based fertilizers in agriculture has disrupted this cycle, leading to groundwater pollution and greenhouse gas emissions. In this study, we have demonstrated the inhibitory effects of plant-derived juglone and related 1,4-naphthoquinones on the nitrification process in . Notably, the inhibition mechanism is elucidated in which 1,4-naphthoquinones interact with hydroxylamine oxidoreductase, disrupting the electron transfer to cytochrome , a physiological electron acceptor. These findings support the notion that phytochemicals can impede nitrification by interfering with the essential electron transfer process in ammonia oxidation. The findings presented in this article offer valuable insights for the development of strategies aimed at the management of nitrification, reduction of fertilizer utilization, and mitigation of greenhouse gas emissions.

PubMed: 38009977
DOI: 10.1128/aem.01291-23

実験手法

X-RAY DIFFRACTION (2.78 Å)