6LXE

DROSHA-DGCR8 complex

6LXE の概要

• エントリーDOI: 10.2210/pdb6lxe/pdb
• EMDBエントリー: 30006
• 分子名称: Ribonuclease 3, Microprocessor complex subunit DGCR8, ZINC ION (3 entities in total)
• 機能のキーワード: ribonuclease, rna binding protein, hydrolase-rna binding protein complex, hydrolase/rna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 287863.80

構造登録者

Jin, W.,Wang, J.,Liu, C.P.,Wang, H.W.,Xu, R.M. (登録日: 2020-02-10, 公開日: 2020-04-15, 最終更新日: 2024-03-27)

主引用文献

Jin, W.,Wang, J.,Liu, C.P.,Wang, H.W.,Xu, R.M.
Structural Basis for pri-miRNA Recognition by Drosha.
Mol.Cell, 78:423-, 2020

PubMed Abstract: A commencing and critical step in miRNA biogenesis involves processing of pri-miRNAs in the nucleus by Microprocessor. An important, but not completely understood, question is how Drosha, the catalytic subunit of Microprocessor, binds pri-miRNAs and correctly specifies cleavage sites. Here we report the cryoelectron microscopy structures of the Drosha-DGCR8 complex with and without a pri-miRNA. The RNA-bound structure provides direct visualization of the tertiary structure of pri-miRNA and shows that a helix hairpin in the extended PAZ domain and the mobile basic (MB) helix in the RNase IIIa domain of Drosha coordinate to recognize the single-stranded to double-stranded junction of RNA, whereas the dsRNA binding domain makes extensive contacts with the RNA stem. Furthermore, the RNA-free structure reveals an autoinhibitory conformation of the PAZ helix hairpin. These findings provide mechanistic insights into pri-miRNA cleavage site selection and conformational dynamics governing pri-miRNA recognition by the catalytic component of Microprocessor.

PubMed: 32220645
DOI: 10.1016/j.molcel.2020.02.024

実験手法

ELECTRON MICROSCOPY (4.2 Å)