6LTJ

Structure of nucleosome-bound human BAF complex

6LTJ の概要

• エントリーDOI: 10.2210/pdb6ltj/pdb
• 関連するPDBエントリー: 6LTH
• EMDBエントリー: 0968 0969 0970 0971 0972 0973 0974
• 分子名称: Histone H3.3, SWI/SNF complex subunit SMARCC2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 1, ... (16 entities in total)
• 機能のキーワード: chromatin remodeler, complex, gene regulation
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 1038933.07

構造登録者

He, S.,Wu, Z.,Tian, Y.,Yu, Z.,Yu, J.,Wang, X.,Li, J.,Liu, B.,Xu, Y. (登録日: 2020-01-22, 公開日: 2020-02-12, 最終更新日: 2024-05-29)

主引用文献

He, S.,Wu, Z.,Tian, Y.,Yu, Z.,Yu, J.,Wang, X.,Li, J.,Liu, B.,Xu, Y.
Structure of nucleosome-bound human BAF complex.
Science, 367:875-881, 2020

PubMed Abstract: Mammalian SWI/SNF family chromatin remodelers, BRG1/BRM-associated factor (BAF) and polybromo-associated BAF (PBAF), regulate chromatin structure and transcription, and their mutations are linked to cancers. The 3.7-angstrom-resolution cryo-electron microscopy structure of human BAF bound to the nucleosome reveals that the nucleosome is sandwiched by the base and the adenosine triphosphatase (ATPase) modules, which are bridged by the actin-related protein (ARP) module. The ATPase motor is positioned proximal to nucleosomal DNA and, upon ATP hydrolysis, engages with and pumps DNA along the nucleosome. The C-terminal α helix of SMARCB1, enriched in positively charged residues frequently mutated in cancers, mediates interactions with an acidic patch of the nucleosome. AT-rich interactive domain-containing protein 1A (ARID1A) and the SWI/SNF complex subunit SMARCC serve as a structural core and scaffold in the base module organization, respectively. Our study provides structural insights into subunit organization and nucleosome recognition of human BAF complex.

PubMed: 32001526
DOI: 10.1126/science.aaz9761

実験手法

ELECTRON MICROSCOPY (3.7 Å)