6LQI

Cryo-EM structure of the mouse Piezo1 isoform Piezo1.1

6LQI の概要

• エントリーDOI: 10.2210/pdb6lqi/pdb
• EMDBエントリー: 0946
• 分子名称: Piezo-type mechanosensitive ion channel component 1 (1 entity in total)
• 機能のキーワード: piezo1.1, mechanogating, mechanotransduction channel, membrane protein, isoform
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 868771.88

構造登録者

Geng, J.,Liu, W.,Zhou, H.,Zhang, T.,Wang, L.,Zhang, M.,Shen, B.,Li, X.,Xiao, B. (登録日: 2020-01-13, 公開日: 2020-03-04, 最終更新日: 2024-03-27)

主引用文献

Geng, J.,Liu, W.,Zhou, H.,Zhang, T.,Wang, L.,Zhang, M.,Li, Y.,Shen, B.,Li, X.,Xiao, B.
A Plug-and-Latch Mechanism for Gating the Mechanosensitive Piezo Channel.
Neuron, 106:438-, 2020

PubMed Abstract: The mechanosensitive Piezo1 and Piezo2 channels convert mechanical force into cation permeation. However, their precise mechanogating and regulatory mechanisms remain elusive. Here, we report that Piezo1 utilizes three lateral ion-conducting portals equipped with physical gates for cooperative gating and splicing regulation. Mutating residues lining the portal converts Piezo1 into an anion-selective channel, demonstrating the portal-based cation-permeating pathway. Intriguingly, the portal is physically blocked with a plug domain, which undergoes alternative splicing in both Piezo1 and Piezo2. The Piezo1 isoform has local openings of the portals, enlarged single-channel conductance and sensitized mechanosensitivity. Remarkably, the three plugs are strategically latched onto the central axis for coordinated gating of the three portals. Disrupting the latching induces three quantal sub-conductance states in Piezo1, but not in the isoform. Together, we propose that Piezo utilizes an elegant plug-and-latch mechanism to physically and coordinately gate the lateral portals through the spliceable plug gates.

PubMed: 32142647
DOI: 10.1016/j.neuron.2020.02.010

実験手法

ELECTRON MICROSCOPY (4.5 Å)