6LOF

Crystal structure of ZsYellow soaked by Cu2+

6LOF の概要

• エントリーDOI: 10.2210/pdb6lof/pdb
• 分子名称: GFP-like fluorescent chromoprotein FP538 (3 entities in total)
• 機能のキーワード: zsyellow, fluorescent protein, yellow
• 由来する生物種: Zoanthus sp. (Green polyp); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 52868.81

構造登録者

Nam, K.H. (登録日: 2020-01-05, 公開日: 2020-01-22, 最終更新日: 2023-11-29)

主引用文献

Kim, I.J.,Xu, Y.,Nam, K.H.
Spectroscopic and Structural Analysis of Cu 2+ -Induced Fluorescence Quenching of ZsYellow.
Biosensors (Basel), 10:-, 2020

PubMed Abstract: Fluorescent proteins exhibit fluorescence quenching by specific transition metals, suggesting their potential as fluorescent protein-based metal biosensors. Each fluorescent protein exhibits unique spectroscopic properties and mechanisms for fluorescence quenching by metals. Therefore, the metal-induced fluorescence quenching analysis of various new fluorescent proteins would be important step towards the development of such fluorescent protein-based metal biosensors. Here, we first report the spectroscopic and structural analysis of the yellow fluorescent protein ZsYellow, following its metal-induced quenching. Spectroscopic analysis showed that ZsYellow exhibited a high degree of fluorescence quenching by Cu. During Cu-induced ZsYellow quenching, fluorescence emission was recovered by adding EDTA. The crystal structure of ZsYellow soaked in Cu solution was determined at a 2.6 Å resolution. The electron density map did not indicate the presence of Cu around the chromophore or the β-barrel surface, which resulted in fluorescence quenching without Cu binding to specific site in ZsYellow. Based on these results, we propose the fluorescence quenching to occur in a distance-dependent manner between the metal and the fluorescent protein, when these components get to a closer vicinity at higher metal concentrations. Our results provide useful insights for future development of fluorescent protein-based metal biosensors.

PubMed: 32210006
DOI: 10.3390/bios10030029

実験手法

X-RAY DIFFRACTION (2.6 Å)