6LNP

Crystal structure of citrate Biosensor

6LNP の概要

• エントリーDOI: 10.2210/pdb6lnp/pdb
• 分子名称: Fusion protein of Green fluorescent protein and Sensor histidine kinase CitA, CITRIC ACID (2 entities in total)
• 機能のキーワード: biosensor, genetically encoded, citrate, fluorescent protein
• 由来する生物種: Aequorea victoria; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90127.42

構造登録者

Wen, Y.,Campbell, R. (登録日: 2019-12-31, 公開日: 2020-05-06, 最終更新日: 2026-03-18)

主引用文献

Zhao, Y.,Shen, Y.,Wen, Y.,Campbell, R.E.
High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate.
Acs Cent.Sci., 6:1441-1450, 2020

PubMed Abstract: Motivated by the growing recognition of citrate as a central metabolite in a variety of biological processes associated with healthy and diseased cellular states, we have developed a series of high-performance genetically encoded citrate biosensors suitable for imaging of citrate concentrations in mammalian cells. The design of these biosensors was guided by structural studies of the citrate-responsive sensor histidine kinase and took advantage of the same conformational changes proposed to propagate from the binding domain to the catalytic domain. Following extensive engineering based on a combination of structure guided mutagenesis and directed evolution, we produced an inverse-response biosensor (Δ/ ≈ 18) designated Citroff1 and a direct-response biosensor (Δ/ ≈ 9) designated Citron1. We report the X-ray crystal structure of Citron1 and demonstrate the utility of both biosensors for qualitative and quantitative imaging of steady-state and pharmacologically perturbed citrate concentrations in live cells.

PubMed: 32875085
DOI: 10.1021/acscentsci.0c00518

実験手法

X-RAY DIFFRACTION (2.993 Å)