6LE4

Crystal structure of cystathionine gamma-lyase from Lactobacillus plantarum complexed with cystathionine

6LE4 の概要

• エントリーDOI: 10.2210/pdb6le4/pdb
• 分子名称: Cystathionine gamma-lyase, (2~{S})-4-[(2~{R})-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]butanoic acid, PHOSPHATE ION, ... (4 entities in total)
• 機能のキーワード: cystathionine gamma-lyase, lyase
• 由来する生物種: Lactobacillus plantarum
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 254486.62

構造登録者

Oda, K.,Matoba, Y. (登録日: 2019-11-24, 公開日: 2020-10-07, 最終更新日: 2023-11-22)

主引用文献

Matoba, Y.,Noda, M.,Yoshida, T.,Oda, K.,Ezumi, Y.,Yasutake, C.,Izuhara-Kihara, H.,Danshiitsoodol, N.,Kumagai, T.,Sugiyama, M.
Catalytic specificity of the Lactobacillus plantarum cystathionine gamma-lyase presumed by the crystallographic analysis.
Sci Rep, 10:14886-14886, 2020

PubMed Abstract: The reverse transsulfuration pathway, which is composed of cystathionine β-synthase (CBS) and cystathionine γ-lyase (CGL), plays a role to synthesize L-cysteine using L-serine and the sulfur atom in L-methionine. A plant-derived lactic acid bacterium Lactobacillus plantarum SN35N has been previously found to harbor the gene cluster encoding the CBS- and CGL-like enzymes. In addition, it has been demonstrated that the L. plantarum CBS can synthesize cystathionine from O-acetyl-L-serine and L-homocysteine. The aim of this study is to characterize the enzymatic functions of the L. plantarum CGL. We have found that the enzyme has the high γ-lyase activity toward cystathionine to generate L-cysteine, together with the β-lyase activity toward L-cystine to generate L-cysteine persulfide. By the crystallographic analysis of the inactive CGL K194A mutant complexed with cystathionine, we have found the residues which recognize the distal amino and carboxyl groups of cystathionine or L-cystine. The PLP-bound substrates at the active site may take either the binding pose for the γ- or β-elimination reaction, with the former being the major reaction in the case of cystathionine.

PubMed: 32913258
DOI: 10.1038/s41598-020-71756-7

実験手法

X-RAY DIFFRACTION (3.1 Å)