6LAB

169 bp nucleosome, harboring cohesive DNA termini, assembled with linker histone H1.0

6LAB の概要

• エントリーDOI: 10.2210/pdb6lab/pdb
• 分子名称: Histone H3.1, CHLORIDE ION, Histone H4, ... (10 entities in total)
• 機能のキーワード: nucleosome, dna-protein complex, dna binding protein, dna binding protein-dna complex, linker histone, h1.0
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 471209.68

構造登録者

Adhireksan, Z.,Sharma, D.,Bao, Q.,Lee, P.L.,Padavattan, S.,Davey, C.A. (登録日: 2019-11-12, 公開日: 2021-02-17, 最終更新日: 2024-04-03)

主引用文献

Adhireksan, Z.,Sharma, D.,Lee, P.L.,Bao, Q.,Padavattan, S.,Shum, W.K.,Davey, G.E.,Davey, C.A.
Engineering nucleosomes for generating diverse chromatin assemblies.
Nucleic Acids Res., 49:e52-e52, 2021

PubMed Abstract: Structural characterization of chromatin is challenging due to conformational and compositional heterogeneity in vivo and dynamic properties that limit achievable resolution in vitro. Although the maximum resolution for solving structures of large macromolecular assemblies by electron microscopy has recently undergone profound increases, X-ray crystallographic approaches may still offer advantages for certain systems. One such system is compact chromatin, wherein the crystalline state recapitulates the crowded molecular environment within the nucleus. Here we show that nucleosomal constructs with cohesive-ended DNA can be designed that assemble into different types of circular configurations or continuous fibers extending throughout crystals. We demonstrate the utility of the method for characterizing nucleosome compaction and linker histone binding at near-atomic resolution but also advance its application for tackling further problems in chromatin structural biology and for generating novel types of DNA nanostructures. We provide a library of cohesive-ended DNA fragment expression constructs and a strategy for engineering DNA-based nanomaterials with a seemingly vast potential variety of architectures and histone chemistries.

PubMed: 33590100
DOI: 10.1093/nar/gkab070

実験手法

X-RAY DIFFRACTION (3.2 Å)