6L8M

WNT DNA promoter mutant G-quadruplex

6L8M の概要

• エントリーDOI: 10.2210/pdb6l8m/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*TP*CP*AP*CP*CP*GP*GP*GP*CP*AP*GP*TP*GP*GP*GP*CP*GP*GP*G)-3') (1 entity in total)
• 機能のキーワード: g-quadruplex, dna promoter, wnt, dna
• 由来する生物種: Homo sapiens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6915.43

構造登録者

Wang, Z.F.,Li, M.H.,Chu, I.T.,Winnerdy, F.R.,Phan, A.T.,Chang, T.C. (登録日: 2019-11-06, 公開日: 2019-12-11, 最終更新日: 2024-05-15)

主引用文献

Wang, Z.F.,Li, M.H.,Chu, I.T.,Winnerdy, F.R.,Phan, A.T.,Chang, T.C.
Cytosine epigenetic modification modulates the formation of an unprecedented G4 structure in the WNT1 promoter.
Nucleic Acids Res., 48:1120-1130, 2020

PubMed Abstract: Time-resolved imino proton nuclear magnetic resonance spectra of the WT22m sequence d(GGGCCACCGGGCAGTGGGCGGG), derived from the WNT1 promoter region, revealed an intermediate G-quadruplex G4(I) structure during K+-induced conformational transition from an initial hairpin structure to the final G4(II) structure. Moreover, a single-base C-to-T mutation at either position C4 or C7 of WT22m could lock the intermediate G4(I) structure without further conformational change to the final G4(II) structure. Surprisingly, we found that the intermediate G4(I) structure is an atypical G4 structure, which differs from a typical hybrid G4 structure of the final G4(II) structure. Further studies of modified cytosine analogues associated with epigenetic regulation indicated that slight modification on a cytosine could modulate G4 structure. A simplified four-state transition model was introduced to describe such conformational transition and disclose the possible mechanism for G4 structural selection caused by cytosine modification.

PubMed: 31912153
DOI: 10.1093/nar/gkz1207

実験手法

SOLUTION NMR