6L8K

Structure of URT1 in complex with UTP

6L8K の概要

• エントリーDOI: 10.2210/pdb6l8k/pdb
• 分子名称: UTP:RNA uridylyltransferase 1, URIDINE 5'-TRIPHOSPHATE (2 entities in total)
• 機能のキーワード: uridylyltransferase, complex, transferase
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43890.74

構造登録者

Lingru, Z. (登録日: 2019-11-06, 公開日: 2020-01-29, 最終更新日: 2023-11-22)

主引用文献

Zhu, L.,Hu, Q.,Cheng, L.,Jiang, Y.,Lv, M.,Liu, Y.,Li, F.,Shi, Y.,Gong, Q.
Crystal structure of Arabidopsis terminal uridylyl transferase URT1.
Biochem.Biophys.Res.Commun., 524:490-496, 2020

PubMed Abstract: 3' uridylation is an essential modification associated with coding and noncoding RNA degradation in eukaryotes. In Arabidopsis, HESO1 was first identified as the major nucleotidyl transferase that uridylates most unmethylated miRNAs, and URT1 was later reported to play a redundant but important role in miRNA uridylation when HESO1 is absent. Two enzymes work sequentially and collaboratively to tail different forms of the same miRNAs in vivo. For mRNA, however, URT1 becomes the main enzyme to uridylate the majority of mRNA and repairs their deadenylated ends to restore the binding site for Poly(A) Binding Protein (PABP). HESO1, on the other hand, targets mostly the mRNAs with very short oligo(A) tails and fails in fulfilling the same task. To understand the structural basis these two functional homologues possess for their different substrate preferences and catalytic behaviors, we first determined the crystal structures of URT1 in the absence and presence of UTP. Our structures, together with functional assay and sequence analysis, indicated that URT1 has a conserved UTP-recognition mechanism analogue to the terminal uridylyl transferases from other species whereas HESO1 may evolve separately to recognize UTP in a different way. Moreover, URT1 N552 may be an important residue in interacting with 3' nucleotide of RNA substrate. The URT1 structure we determined represents the first structure of uridylyl transferase from plants, shedding light on the mechanisms of URT1/HESO1-dependent RNA metabolism.

PubMed: 32008746
DOI: 10.1016/j.bbrc.2020.01.124

実験手法

X-RAY DIFFRACTION (2.999 Å)