6L80

Crystal structure of pombe Mod21 N-terminus and Mozart1

6L80 の概要

• エントリーDOI: 10.2210/pdb6l80/pdb
• 分子名称: Gamma-tubulin complex subunit mod21, Mitotic-spindle organizing protein 1 (3 entities in total)
• 機能のキーワード: gamma tubulin complex, microprotien, microtubule, translation
• 由来する生物種: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 48492.05

構造登録者

Huang, T.L.,Wang, H.J.,Wang, S.W.,Hsia, K.C. (登録日: 2019-11-03, 公開日: 2020-07-15, 最終更新日: 2024-03-27)

主引用文献

Huang, T.L.,Wang, H.J.,Chang, Y.C.,Wang, S.W.,Hsia, K.C.
Promiscuous Binding of Microprotein Mozart1 to gamma-Tubulin Complex Mediates Specific Subcellular Targeting to Control Microtubule Array Formation.
Cell Rep, 31:107836-107836, 2020

PubMed Abstract: How γ-tubulin ring complex (γ-TuRC), a master template for microtubule nucleation, is spatially and temporally regulated for the assembly of new microtubule arrays remains unclear. Here, we report that an evolutionarily conserved microprotein, Mozart1 (Mzt1), regulates subcellular targeting and microtubule formation activity of γ-TuRC at different cell cycle stages. Crystal structures of protein complexes demonstrate that Mzt1 promiscuously interacts with the N-terminal domains of multiple γ-tubulin complex protein subunits in γ-TuRC via an intercalative binding mode. Genetic- and microscopy-based analyses show that promiscuous binding of Mzt1 in γ-TuRC controls specific subcellular localization of γ-TuRC to modulate microtubule nucleation and stabilization in fission yeast. Moreover, we find Mzt1-independent targeting of γ-TuRC to be crucial for mitotic spindle assembly, demonstrating the cell-cycle-dependent regulation and function of γ-TuRC. Our findings reveal a microprotein-mediated regulatory mechanism underlying microtubule cytoskeleton formation, whereby Mzt1 binding promiscuity confers localization specificity on the multi-protein complex γ-TuRC.

PubMed: 32610137
DOI: 10.1016/j.celrep.2020.107836

実験手法

X-RAY DIFFRACTION (2.00049753238 Å)