6L7Q

Crystal structure of a hypothetical protein PYCH_01220 derived from Pyrococcus yayanosii

Summary for 6L7Q

• Entry DOI: 10.2210/pdb6l7q/pdb
• Descriptor: hypothetical protein (2 entities in total)
• Functional Keywords: cytosolic protein, unknown function, hypothetical protein
• Biological source: Pyrococcus yayanosii (strain CH1 / JCM 16557)
• Total number of polymer chains: 1
• Total formula weight: 17012.14

Authors

Jeon, J.-H.,Noh, H.,Oh, B.-H. (deposition date: 2019-11-02, release date: 2020-11-04, Last modification date: 2024-03-27)

Primary citation

Noh, H.,Jeon, J.H.,Kim, Y.G.,Oh, B.H.
Crystal structure of PYCH_01220 from Pyrococcus yayanosii potentially involved in binding nucleic acid.
Proteins, 89:468-472, 2021

PubMed Abstract: We report the crystal structure of PYCH_01220, a hypothetical protein in Pyrococcus yayanosii CH1. This protein is composed of two domains, named Domain A and Domain B. While Domain B is not significantly homologous to known protein structures, Domain A is structurally analogous to the C-terminal ribonuclease domain of Escherichia coli colicin D. Domain A has a positively charged surface patch rendered by 13 basic residues, eight arginine or lysine residues of which are evolutionarily conserved. Electrophoretic mobility shift assays showed that PYCH_01220 binds to DNA, and charge-inversion mutations on this patch negatively affect the DNA binding, suggesting that the function of PYCH_01220 might involve nucleic acid-binding via the positively charged patch.

PubMed: 33236809
DOI: 10.1002/prot.26029

Experimental method

X-RAY DIFFRACTION (1.16 Å)