6L7Q

Crystal structure of a hypothetical protein PYCH_01220 derived from Pyrococcus yayanosii

6L7Q の概要

• エントリーDOI: 10.2210/pdb6l7q/pdb
• 分子名称: hypothetical protein (2 entities in total)
• 機能のキーワード: cytosolic protein, unknown function, hypothetical protein
• 由来する生物種: Pyrococcus yayanosii (strain CH1 / JCM 16557)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17012.14

構造登録者

Jeon, J.-H.,Noh, H.,Oh, B.-H. (登録日: 2019-11-02, 公開日: 2020-11-04, 最終更新日: 2024-03-27)

主引用文献

Noh, H.,Jeon, J.H.,Kim, Y.G.,Oh, B.H.
Crystal structure of PYCH_01220 from Pyrococcus yayanosii potentially involved in binding nucleic acid.
Proteins, 89:468-472, 2021

PubMed Abstract: We report the crystal structure of PYCH_01220, a hypothetical protein in Pyrococcus yayanosii CH1. This protein is composed of two domains, named Domain A and Domain B. While Domain B is not significantly homologous to known protein structures, Domain A is structurally analogous to the C-terminal ribonuclease domain of Escherichia coli colicin D. Domain A has a positively charged surface patch rendered by 13 basic residues, eight arginine or lysine residues of which are evolutionarily conserved. Electrophoretic mobility shift assays showed that PYCH_01220 binds to DNA, and charge-inversion mutations on this patch negatively affect the DNA binding, suggesting that the function of PYCH_01220 might involve nucleic acid-binding via the positively charged patch.

PubMed: 33236809
DOI: 10.1002/prot.26029

実験手法

X-RAY DIFFRACTION (1.16 Å)