6L68

X-ray structure of human galectin-10 in complex with D-allose

Summary for 6L68

• Entry DOI: 10.2210/pdb6l68/pdb
• Descriptor: Galectin-10, beta-D-allopyranose (3 entities in total)
• Functional Keywords: beta-sandwich structure, lectin, sugar binding protein
• Biological source: Homo sapiens (Human)
• Total number of polymer chains: 1
• Total formula weight: 17004.33

Authors

Kamitori, S. (deposition date: 2019-10-28, release date: 2020-03-04, Last modification date: 2023-11-22)

Primary citation

Itoh, A.,Nonaka, Y.,Nakakita, S.I.,Yoshida, H.,Nishi, N.,Nakamura, T.,Kamitori, S.
Structures of human galectin-10/monosaccharide complexes demonstrate potential of monosaccharides as effectors in forming Charcot-Leyden crystals.
Biochem.Biophys.Res.Commun., 2020

PubMed Abstract: The galectins are a family of β-galactoside-specific animal lectins, and have attracted much attention as novel regulators of the immune system. Galectin-10 is well-expressed in eosinophils, and spontaneously forms Charcot-Leyden crystals (CLCs), during prolonged eosinophilic inflammatory reactions, which are frequently observed in eosinophilic diseases. Although biochemical and structural characterizations of galectin-10 have been done, its biological role and molecular mechanism are still unclear, and few X-ray structures of galectin-10 in complex with monosaccharides/oligosaccharides have been reported. Here, X-ray structures of galectin-10 in complexes with seven monosaccharides are presented with biochemical analyses to detect interactions of galectin-10 with monosaccharides/oligosaccharides. Galectin-10 forms a homo-dimer in the face-to-face orientation, and the monosaccharides bind to the carbohydrate recognition site composed of amino acid residues from two galectin-10 molecules of dimers, suggesting that galectin-10 dimer likely captures the monosaccharides in solution and in vivo. d-Glucose, d-allose, d-arabinose, and D-N-acetylgalactosamine bind to the interfaces between galectin-10 dimers in crystals, and they affect the stability of molecular packing in crystals, leading to easy-dissolving of CLCs, and/or inhibiting the formation of CLCs. These monosaccharides may serve as effectors of G10 to form CLCs in vivo.

PubMed: 32081418
DOI: 10.1016/j.bbrc.2020.02.037

Experimental method

X-RAY DIFFRACTION (1.92 Å)