6L33

Crystal structure of the regulatory domain of MexT, a transcriptional activator in Pseudomonas aeruginosa

Summary for 6L33

• Entry DOI: 10.2210/pdb6l33/pdb
• Descriptor: MexT protein, SULFATE ION (3 entities in total)
• Functional Keywords: lysr-type transcriptional regulator, antibiotic resistance, regulatory domain, transcription
• Biological source: Pseudomonas aeruginosa
• Total number of polymer chains: 4
• Total formula weight: 93256.16

Authors

Kim, S.,Ha, N.-C. (deposition date: 2019-10-08, release date: 2019-10-30, Last modification date: 2024-10-30)

Primary citation

Kim, S.,Kim, S.H.,Ahn, J.,Jo, I.,Lee, Z.W.,Choi, S.H.,Ha, N.
Crystal Structure of the Regulatory Domain of MexT, a Transcriptional Activator of the MexEFOprN Efflux Pump inPseudomonas aeruginosa.
Mol.Cells, 42:850-857, 2019

PubMed Abstract: The Gram-negative opportunistic pathogen, Pseudomonas aeruginosa , has multiple multidrug efflux pumps. MexT, a LysR-type transcriptional regulator, functions as a transcriptional activator of the MexEF-OprN efflux system. MexT consists of an N-terminal DNA-binding domain and a C-terminal regulatory domain (RD). Little is known regarding MexT ligands and its mechanism of activation. We elucidated the crystal structure of the MexT RD at 2.0 Å resolution. The structure comprised two protomer chains in a dimeric arrangement. MexT possessed an arginine-rich region and a hydrophobic patch lined by a variable loop, both of which are putative ligand-binding sites. The three-dimensional structure of MexT provided clues to the interacting ligand structure. A DNase I footprinting assay of full-length MexT identified two MexT-binding sequence in the promoter. Our findings enhance the understanding of the regulation of MexT-dependent activation of efflux pumps.

PubMed: 31722511
DOI: 10.14348/molcells.2019.0168

Experimental method

X-RAY DIFFRACTION (2 Å)