6KW4

The ClassB RSC-Nucleosome Complex

これはPDB形式変換不可エントリーです。

6KW4 の概要

• エントリーDOI: 10.2210/pdb6kw4/pdb
• EMDBエントリー: 0778
• 分子名称: Histone H3.2, Chromatin structure-remodeling complex subunit RSC9, Chromatin structure-remodeling complex protein RSC6, ... (22 entities in total)
• 機能のキーワード: chromatin remodeler, swi/snf family, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 1598362.90

構造登録者

Ye, Y.P.,Wu, H.,Chen, K.J.,Verma, N.,Cairns, B.,Gao, N.,Chen, Z.C. (登録日: 2019-09-06, 公開日: 2019-11-13, 最終更新日: 2024-05-29)

主引用文献

Ye, Y.,Wu, H.,Chen, K.,Clapier, C.R.,Verma, N.,Zhang, W.,Deng, H.,Cairns, B.R.,Gao, N.,Chen, Z.
Structure of the RSC complex bound to the nucleosome.
Science, 366:838-843, 2019

PubMed Abstract: The RSC complex remodels chromatin structure and regulates gene transcription. We used cryo-electron microscopy to determine the structure of yeast RSC bound to the nucleosome. RSC is delineated into the adenosine triphosphatase motor, the actin-related protein module, and the substrate recruitment module (SRM). RSC binds the nucleosome mainly through the motor, with the auxiliary subunit Sfh1 engaging the H2A-H2B acidic patch to enable nucleosome ejection. SRM is organized into three substrate-binding lobes poised to bind their respective nucleosomal epitopes. The relative orientations of the SRM and the motor on the nucleosome explain the directionality of DNA translocation and promoter nucleosome repositioning by RSC. Our findings shed light on RSC assembly and functionality, and they provide a framework to understand the mammalian homologs BAF/PBAF and the Sfh1 ortholog INI1/BAF47, which are frequently mutated in cancers.

PubMed: 31672915
DOI: 10.1126/science.aay0033

実験手法

ELECTRON MICROSCOPY (7.55 Å)