6KVN

Crystal structure of chloroplast resolvase

6KVN の概要

• エントリーDOI: 10.2210/pdb6kvn/pdb
• 分子名称: NtMOC1 (2 entities in total)
• 機能のキーワード: chloroplast, resolvase, plant protein
• 由来する生物種: Nicotiana tabacum (Common tobacco)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18605.33

構造登録者

Yan, J.J.,Hong, S.X.,Guan, Z.Y.,Yin, P. (登録日: 2019-09-05, 公開日: 2020-04-08, 最終更新日: 2024-03-27)

主引用文献

Yan, J.,Hong, S.,Guan, Z.,He, W.,Zhang, D.,Yin, P.
Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.
Nat Commun, 11:1417-1417, 2020

PubMed Abstract: Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.

PubMed: 32184398
DOI: 10.1038/s41467-020-15242-8

実験手法

X-RAY DIFFRACTION (2.02 Å)