6KOQ

Mycobacterium tuberculosis initial transcription complex comprising sigma H and 5'-OH RNA of 10 nt

6KOQ の概要

• エントリーDOI: 10.2210/pdb6koq/pdb
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, DNA-directed RNA polymerase subunit beta, ... (11 entities in total)
• 機能のキーワード: transcription
• 由来する生物種: Mycobacterium tuberculosis H37Rv; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 410401.67

構造登録者

Li, L.,Zhang, Y. (登録日: 2019-08-12, 公開日: 2020-03-11, 最終更新日: 2023-11-22)

主引用文献

Li, L.,Molodtsov, V.,Lin, W.,Ebright, R.H.,Zhang, Y.
RNA extension drives a stepwise displacement of an initiation-factor structural module in initial transcription.
Proc.Natl.Acad.Sci.USA, 117:5801-5809, 2020

PubMed Abstract: All organisms-bacteria, archaea, and eukaryotes-have a transcription initiation factor that contains a structural module that binds within the RNA polymerase (RNAP) active-center cleft and interacts with template-strand single-stranded DNA (ssDNA) in the immediate vicinity of the RNAP active center. This transcription initiation-factor structural module preorganizes template-strand ssDNA to engage the RNAP active center, thereby facilitating binding of initiating nucleotides and enabling transcription initiation from initiating mononucleotides. However, this transcription initiation-factor structural module occupies the path of nascent RNA and thus presumably must be displaced before or during initial transcription. Here, we report four sets of crystal structures of bacterial initially transcribing complexes that demonstrate and define details of stepwise, RNA-extension-driven displacement of the "σ-finger" of the bacterial transcription initiation factor σ. The structures reveal that-for both the primary σ-factor and extracytoplasmic (ECF) σ-factors, and for both 5'-triphosphate RNA and 5'-hydroxy RNA-the "σ-finger" is displaced in stepwise fashion, progressively folding back upon itself, driven by collision with the RNA 5'-end, upon extension of nascent RNA from ∼5 nt to ∼10 nt.

PubMed: 32127479
DOI: 10.1073/pnas.1920747117

実験手法

X-RAY DIFFRACTION (3.353 Å)