6KOJ

Crystal structure of SNX11-PXe domain in complex with PI(3,5)P2

6KOJ の概要

• エントリーDOI: 10.2210/pdb6koj/pdb
• 関連するPDBエントリー: 6KOI
• 分子名称: Sorting nexin-11, [(2~{R})-2-butanoyloxy-3-[oxidanyl-[(2~{R},3~{R},5~{S},6~{R})-2,4,6-tris(oxidanyl)-3,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] butanoate (3 entities in total)
• 機能のキーワード: sorting nexin, protein transport, phox-homology domain
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35165.00

構造登録者

Xu, T.,Liu, J. (登録日: 2019-08-11, 公開日: 2020-07-08, 最終更新日: 2023-11-22)

主引用文献

Xu, T.,Gan, Q.,Wu, B.,Yin, M.,Xu, J.,Shu, X.,Liu, J.
Molecular Basis for PI(3,5)P2Recognition by SNX11, a Protein Involved in Lysosomal Degradation and Endosome Homeostasis Regulation.
J.Mol.Biol., 432:4750-4761, 2020

PubMed Abstract: Phosphatidylinositol 3,5-bisphosphate (PI(3,5)P) is an essential phosphoinositide required for endosome homeostasis and sorting for lysosomal degradation; however, the underlying mechanisms, especially in mammals, remain elusive or unexplored. Here we determined a structure of PI(3,5)P bound to Sorting Nexin 11 (SNX11) with an opened PPII-C loop. We also obtained an SNX11 structure with its PPII-C in "closed" form that serves as a potential PI3P-binding model. In addition, our results reveal that SNX11 can interact with the V1D subunit of vacuolar H-ATPase (V-ATPase), which provides a link between PI(3,5)P and human V-ATPase and further evidence for their roles in the endosome homeostasis regulation. Lastly, a new apo-form structure of SNX11, combined with molecular dynamics (MD) studies, indicates that the α5 helix can unfold from the PX domain of SNX11 when targeting the membrane or interacting with its partner. Taken together, these findings identify a novel PI(3,5)P effector, which will shed light on the PIs recognizing mechanism and the understanding of the downstream sorting events triggered by different PI binding.

PubMed: 32561432
DOI: 10.1016/j.jmb.2020.06.010

実験手法

X-RAY DIFFRACTION (2.14 Å)