6KNB

PolD-PCNA-DNA (form A)

6KNB の概要

• エントリーDOI: 10.2210/pdb6knb/pdb
• EMDBエントリー: 0723
• 分子名称: DNA polymerase II small subunit, DNA polymerase D DP2 (DNA polymerase II large) subunit, DNA polymerase sliding clamp 1, ... (7 entities in total)
• 機能のキーワード: family d polymerase, pcna, switch hook, replication, replication-dna complex, replication/dna
• 由来する生物種: Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 314767.07

構造登録者

Mayanagi, K.,Oki, K.,Miyazaki, N.,Ishino, S.,Yamagami, T.,Iwasaki, K.,Kohda, D.,Morikawa, K.,Shirai, T.,Ishino, Y. (登録日: 2019-08-05, 公開日: 2020-08-05, 最終更新日: 2021-02-17)

主引用文献

Mayanagi, K.,Oki, K.,Miyazaki, N.,Ishino, S.,Yamagami, T.,Morikawa, K.,Iwasaki, K.,Kohda, D.,Shirai, T.,Ishino, Y.
Two conformations of DNA polymerase D-PCNA-DNA, an archaeal replisome complex, revealed by cryo-electron microscopy.
Bmc Biol., 18:152-152, 2020

PubMed Abstract: DNA polymerase D (PolD) is the representative member of the D family of DNA polymerases. It is an archaea-specific DNA polymerase required for replication and unrelated to other known DNA polymerases. PolD consists of a heterodimer of two subunits, DP1 and DP2, which contain catalytic sites for 3'-5' editing exonuclease and DNA polymerase activities, respectively, with both proteins being mutually required for the full activities of each enzyme. However, the processivity of the replicase holoenzyme has additionally been shown to be enhanced by the clamp molecule proliferating cell nuclear antigen (PCNA), making it crucial to elucidate the interaction between PolD and PCNA on a structural level for a full understanding of its functional relevance. We present here the 3D structure of a PolD-PCNA-DNA complex from Thermococcus kodakarensis using single-particle cryo-electron microscopy (EM).

PubMed: 33115459
DOI: 10.1186/s12915-020-00889-y

実験手法

ELECTRON MICROSCOPY (6.9 Å)