6KL4

Crystal structure of MavC-UBE2N-Ub

6KL4 の概要

• エントリーDOI: 10.2210/pdb6kl4/pdb
• 分子名称: MavC, Ubiquitin-conjugating enzyme E2 N, Ub (3 entities in total)
• 機能のキーワード: legionella pneumophila virulence factors complex, transferase
• 由来する生物種: Legionella pneumophila; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 68829.25

構造登録者

Ouyang, S.,Guan, H. (登録日: 2019-07-29, 公開日: 2020-04-15, 最終更新日: 2024-03-27)

主引用文献

Guan, H.,Fu, J.,Yu, T.,Wang, Z.X.,Gan, N.,Huang, Y.,Perculija, V.,Li, Y.,Luo, Z.Q.,Ouyang, S.
Molecular Basis of Ubiquitination Catalyzed by the Bacterial Transglutaminase MavC.
Adv Sci, 7:2000871-2000871, 2020

PubMed Abstract: The effector MavC is a transglutaminase that carries out atypical ubiquitination of the host ubiquitin (Ub)-conjugation enzyme UBE2N by catalyzing the formation of an isopeptide bond between Gln40 and Lys92, which leads to inhibition of signaling in the NF-κB pathway. In the absence of UBE2N, MavC deamidates Ub at Gln40 or catalyzes self-ubiquitination. However, the mechanisms underlying these enzymatic activities of MavC are poorly understood at the molecular level. This study reports the structure of the MavC-UBE2N-Ub ternary complex representing a snapshot of MavC-catalyzed crosslinking of UBE2N and Ub, which reveals the way by which UBE2N-Ub binds to the Insertion and Tail domains of MavC. Based on the structural and experimental data, the catalytic mechanism for the deamidase and transglutaminase activities of MavC is proposed. Finally, by comparing the structures of MavC and MvcA, the homologous protein that reverses MavC-induced UBE2N ubiquitination, several essential regions and two key residues (Trp255 and Phe268) responsible for their respective enzymatic activities are identified. The results provide insights into the mechanisms for substrate recognition and ubiquitination mediated by MavC as well as explanations for the opposite activity of MavC and MvcA in terms of regulation of UBE2N ubiquitination.

PubMed: 32596129
DOI: 10.1002/advs.202000871

実験手法

X-RAY DIFFRACTION (2.85 Å)