6KKK

Crystal structure of Drug:Proton Antiporter-1 (DHA1) Family SotB, in the inward open conformation (H115A mutant)

6KKK の概要

• エントリーDOI: 10.2210/pdb6kkk/pdb
• 分子名称: Sugar efflux transporter, nonyl beta-D-glucopyranoside (2 entities in total)
• 機能のキーワード: mfs transporter, transport protein
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 137190.96

構造登録者

Xiao, Q.J.,Deng, D. (登録日: 2019-07-26, 公開日: 2020-07-29, 最終更新日: 2024-05-29)

主引用文献

Xiao, Q.,Sun, B.,Zhou, Y.,Wang, C.,Guo, L.,He, J.,Deng, D.
Visualizing the nonlinear changes of a drug-proton antiporter from inward-open to occluded state.
Biochem.Biophys.Res.Commun., 534:272-278, 2021

PubMed Abstract: Drug-proton antiporters (DHA) play an important role in multi-drug resistance, utilizing the proton-motive force to drive the expulsion of toxic molecules, including antibiotics and drugs. DHA transporters belong to the major facilitator superfamily (MFS), members of which deliver substrates by utilizing the alternating access model of transport. However, the transport process is still elusive. Here, we report the structures of SotB, a member of DHA1 family (TCDB: 2.A.1.2) from Escherichia coli. Four crystal structures of SotB were captured in different conformations, including substrate-bound occluded, inward-facing, and inward-open states. Comparisons between the four structures reveal nonlinear rigid-body movements of alternating access during the state transition from inward-open to occluded conformation. This work not only reveals the conformational dynamics of SotB but also deepens our understanding of the alternating access mechanism of MFS transporters.

PubMed: 33280821
DOI: 10.1016/j.bbrc.2020.11.096

実験手法

X-RAY DIFFRACTION (3.5 Å)