6KJI

Crystal structure of PsoF with SAH

6KJI の概要

• エントリーDOI: 10.2210/pdb6kji/pdb
• 分子名称: Dual-functional monooxygenase/methyltransferase psoF, S-ADENOSYL-L-HOMOCYSTEINE, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: metyltransferase, oxidoreductase
• 由来する生物種: Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 126925.73

構造登録者

Hara, K.,Hashimoto, H.,Matsushita, T.,Tsunematsu, Y.,Watanabe, K. (登録日: 2019-07-22, 公開日: 2019-09-18, 最終更新日: 2023-11-22)

主引用文献

Kishimoto, S.,Tsunematsu, Y.,Matsushita, T.,Hara, K.,Hashimoto, H.,Tang, Y.,Watanabe, K.
Functional and Structural Analyses oftrans C-Methyltransferase in Fungal Polyketide Biosynthesis.
Biochemistry, 58:3933-3937, 2019

PubMed Abstract: Biosynthesis of certain fungal polyketide-peptide synthetases involves -methyltransferase activity that adds one or more -adenosyl-l-methionine-derived methyl groups to the carbon framework. The previously reported PsoF-MT, the stand-alone -methyltransferase (MT) from the pseurotin biosynthetic pathway that exists as a domain within a trifunctional didomain enzyme PsoF, was characterized crystallographically and kinetically using mutants with substrate analogs to understand how a -acting -MT works and compare it to known polyketide synthase-associated -MTs. This study identified key active-site residues involved in catalysis and substrate recognition, which led us to propose the mechanism of -methylation and substrate specificity determinants in PsoF-MT.

PubMed: 31486637
DOI: 10.1021/acs.biochem.9b00702

実験手法

X-RAY DIFFRACTION (1.99 Å)