6KC8

Crystal structure of WT Nme1Cas9 in complex with sgRNA and target DNA (ATATGATT PAM) in post-cleavage state

6KC8 の概要

• エントリーDOI: 10.2210/pdb6kc8/pdb
• 分子名称: CRISPR-associated endonuclease Cas9, sgRNA, DNA (5'-D(P*AP*AP*GP*TP*TP*AP*AP*AP*TP*AP*GP*CP*AP*GP*AP*GP*TP*GP*AP*CP*C)-3'), ... (6 entities in total)
• 機能のキーワード: crispr-cas9, nmecas9, nme1cas9, hydrolase, ternary complex, hydrolase-rna-dna complex, hydrolase/rna/dna
• 由来する生物種: Neisseria meningitidis 8013; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 181913.64

構造登録者

Sun, W.,Yang, J.,Cheng, Z.,Liu, C.,Wang, K.,Huang, X.,Wang, Y. (登録日: 2019-06-27, 公開日: 2019-11-06, 最終更新日: 2023-11-22)

• 主引用文献: Sun, W.,Yang, J.,Cheng, Z.,Amrani, N.,Liu, C.,Wang, K.,Ibraheim, R.,Edraki, A.,Huang, X.,Wang, M.,Wang, J.,Liu, L.,Sheng, G.,Yang, Y.,Lou, J.,Sontheimer, E.J.,Wang, Y.; Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States.; Mol.Cell, 76:938-, 2019; PubMed: 31668930; DOI: 10.1016/j.molcel.2019.09.025

実験手法

X-RAY DIFFRACTION (2.9 Å)