6K7C

Dimeric Shewanella violacea cytochrome c5

6K7C の概要

• エントリーDOI: 10.2210/pdb6k7c/pdb
• 分子名称: Soluble cytochrome cA, HEME C, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: electron transport
• 由来する生物種: Shewanella violacea
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17325.22

構造登録者

Yang, H.,Yamanaka, M.,Nagao, S.,Yasuhara, K.,Shibata, N.,Higuchi, Y.,Hirota, S. (登録日: 2019-06-07, 公開日: 2019-09-04, 最終更新日: 2024-11-13)

主引用文献

Yang, H.,Yamanaka, M.,Nagao, S.,Yasuhara, K.,Shibata, N.,Higuchi, Y.,Hirota, S.
Protein surface charge effect on 3D domain swapping in cells for c-type cytochromes.
Biochim Biophys Acta Proteins Proteom, 1867:140265-140265, 2019

PubMed Abstract: Many c-type cytochromes (cyts) can form domain-swapped oligomers. The positively charged Hydrogenobacter thermophilus (HT) cytochrome (cyt) c forms domain-swapped oligomers during expression in the Escherichia coli (E. coli) expression system, but the factors influencing the oligomerization remain unrevealed. Here, we found that the dimer of the negatively charged Shewanella violacea (SV) cyt c exhibits a domain-swapped structure, in which the N-terminal helix is exchanged between protomers, similar to the structures of the HT cyt c and Pseudomonas aeruginosa (PA) cyt c domain-swapped dimers. Positively charged horse cyt c and HT cyt c domain swapped during expression in E. coli, whereas negatively charged PA cyt c and SV cyt c did not. Oligomers were formed during expression in E. coli for HT cyt c attached to either a co- or post-translational signal peptide for transportation through the cytoplasm membrane, but not for PA cyt c attached to either signal peptide. HT cyt c formed oligomers in E. coli in the presence and absence of rare codons. More oligomers were obtained from the in vitro folding of horse cyt c and HT cyt c by the addition of negatively charged liposomes during folding, whereas the amount of oligomers for the in vitro folding of PA cyt c and SV cyt c did not change significantly by the addition. These results indicate that the protein surface charge affects the oligomerization of c-type cyts in cells; positively charged c-type cyts assemble on a negatively charged membrane, inducing formation of domain-swapped oligomers during folding.

PubMed: 31437585
DOI: 10.1016/j.bbapap.2019.140265

実験手法

X-RAY DIFFRACTION (1.15 Å)