6K4D

Ancestral luciferase AncLamp in complex with ATP and D-luciferin

6K4D の概要

• エントリーDOI: 10.2210/pdb6k4d/pdb
• 分子名称: Ancestral luciferase AncLamp, [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] (4S)-2-(6-oxidanyl-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate, (4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylic acid, ... (4 entities in total)
• 機能のキーワード: luciferase, bioluminescence, ancestral protein, molecular evolution, oxidoreductase
• 由来する生物種: Lampyridae
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61264.22

構造登録者

Oba, Y.,Konishi, K.,Yano, D.,Kato, D.,Shirai, T. (登録日: 2019-05-23, 公開日: 2020-05-27, 最終更新日: 2023-11-22)

主引用文献

Oba, Y.,Konishi, K.,Yano, D.,Shibata, H.,Kato, D.,Shirai, T.
Resurrecting the ancient glow of the fireflies.
Sci Adv, 6:-, 2020

PubMed Abstract: The color of firefly bioluminescence is determined by the structure of luciferase. Firefly luciferase genes have been isolated from more than 30 species, producing light ranging in color from green to orange-yellow. Here, we reconstructed seven ancestral firefly luciferase genes, characterized the enzymatic properties of the recombinant proteins, and determined the crystal structures of the gene from ancestral Lampyridae. Results showed that the synthetic luciferase for the last common firefly ancestor exhibited green light caused by a spatial constraint on the luciferin molecule in enzyme, while fatty acyl-CoA synthetic activity, an original function of firefly luciferase, was diminished in exchange. All known firefly species are bioluminescent in the larvae, with a common ancestor arising approximately 100 million years ago. Combined, our findings propose that, within the mid-Cretaceous forest, the common ancestor of fireflies evolved green light luciferase via trade-off of the original function, which was likely aposematic warning display against nocturnal predation.

PubMed: 33268373
DOI: 10.1126/sciadv.abc5705

実験手法

X-RAY DIFFRACTION (1.7 Å)