6K3A

Crystal structure of human PCNA in complex with DNMT1 PIP box motif.

6K3A の概要

• エントリーDOI: 10.2210/pdb6k3a/pdb
• 分子名称: Proliferating cell nuclear antigen, Peptide from DNA (cytosine-5)-methyltransferase 1 (3 entities in total)
• 機能のキーワード: dnmt1, pcna, dna methylation, pip box, replication
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 93676.56

構造登録者

Jimenji, T.,Kori, S.,Arita, K. (登録日: 2019-05-17, 公開日: 2019-06-26, 最終更新日: 2023-11-22)

主引用文献

Jimenji, T.,Matsumura, R.,Kori, S.,Arita, K.
Structure of PCNA in complex with DNMT1 PIP box reveals the basis for the molecular mechanism of the interaction.
Biochem.Biophys.Res.Commun., 516:578-583, 2019

PubMed Abstract: DNMT1 is a C5-DNA methyltransferase that plays a pivotal role in DNA methylation maintenance. During early and mid S-phase, DNMT1 accumulates at DNA replication sites by binding to proliferating cell nuclear antigen (PCNA), an essential factor for DNA replication, through a PIP box motif. However, the molecular mechanism by which the DNMT1 PIP box motif binds to PCNA remains unclear. Here, we report the crystal structure of PCNA bound to DNMT1 PIP box peptide. The structure reveals the detailed interaction between PCNA and DNMT1 PIP box; conserved glutamine and hydrophobic/aromatic residues in the PIP box are recognized by the Q- and hydrophobic pockets of PCNA, respectively. The structure also shows novel intramolecular interactions within the PIP box motif, which stabilize the helix conformation in the PIP box. Our data provide structural insight into the recruitment of DNMT1 to replication sites by PCNA.

PubMed: 31235252
DOI: 10.1016/j.bbrc.2019.06.060

実験手法

X-RAY DIFFRACTION (2.3 Å)