6K2J

Crystal Structure of the DNA Complex of C. crescentus GapR

6K2J の概要

• エントリーDOI: 10.2210/pdb6k2j/pdb
• 関連するPDBエントリー: 6JYK
• 分子名称: UPF0335 protein CCNA_03428, 10A DNA_front, 10A DNA_reverse, ... (4 entities in total)
• 機能のキーワード: c. crescentus, gapr/dna, naps, dna binding protein, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Caulobacter vibrioides (strain NA1000 / CB15N); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 54577.42

構造登録者

Xia, B.,Huang, Q. (登録日: 2019-05-14, 公開日: 2020-08-05, 最終更新日: 2024-03-27)

主引用文献

Huang, Q.,Duan, B.,Dong, X.,Fan, S.,Xia, B.
GapR binds DNA through dynamic opening of its tetrameric interface.
Nucleic Acids Res., 48:9372-9386, 2020

PubMed Abstract: GapR is a nucleoid-associated protein that is an essential regulator of chromosome replication in the cell cycle model Caulobacter crescentus. Here, we demonstrate that free GapR is a homotetramer, but not a dimer as previously reported (Guo et al., Cell 175: 583-597, 2018). We have determined the crystal structure of GapR in complex with a 10-bp A-tract DNA, which has an open tetrameric conformation, different from the closed clamp conformation in the previously reported crystal structure of GapR/DNA complex. The free GapR adopts multiple conformations in dynamic exchange equilibrium, with the major conformation resembling the closed tetrameric conformation, while the open tetrameric conformation is a representative of minor conformers. As it is impossible for the circular genomic DNA to get into the central DNA binding tunnel of the major conformation, we propose that GapR initially binds DNA through the open conformation, and then undergoes structural rearrangement to form the closed conformation which fully encircles the DNA. GapR prefers to bind DNA with 10-bp consecutive A/T base pairs nonselectively (Kd ∼12 nM), while it can also bind GC-rich DNA sequence with a reasonable affinity of about 120 nM. Besides, our results suggest that GapR binding results in widening the minor groove of AT-rich DNA, instead of overtwisting DNA.

PubMed: 32756896
DOI: 10.1093/nar/gkaa644

実験手法

X-RAY DIFFRACTION (2.4 Å)