6JZH

Structure of human A2A adenosine receptor in complex with ZM241385 obtained from SFX experiments under atmospheric pressure

Summary for 6JZH

• Entry DOI: 10.2210/pdb6jzh/pdb
• Descriptor: Adenosine receptor A2a,Soluble cytochrome b562,Adenosine receptor A2a, SODIUM ION, 4-{2-[(7-amino-2-furan-2-yl[1,2,4]triazolo[1,5-a][1,3,5]triazin-5-yl)amino]ethyl}phenol, ... (6 entities in total)
• Functional Keywords: x-ray free-electron lasers room-temperature g-protein-coupled receptor, membrane protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 1
• Total formula weight: 56875.35

Authors

Nango, E.,Shimamura, T.,Nakane, T.,Yamanaka, Y.,Mori, C.,Kimura, K.T.,Fujiwara, T.,Tanaka, T.,Iwata, S. (deposition date: 2019-05-02, release date: 2019-10-30, Last modification date: 2024-10-16)

Primary citation

Shimazu, Y.,Tono, K.,Tanaka, T.,Yamanaka, Y.,Nakane, T.,Mori, C.,Terakado Kimura, K.,Fujiwara, T.,Sugahara, M.,Tanaka, R.,Doak, R.B.,Shimamura, T.,Iwata, S.,Nango, E.,Yabashi, M.
High-viscosity sample-injection device for serial femtosecond crystallography at atmospheric pressure.
J.Appl.Crystallogr., 52:1280-1288, 2019

PubMed Abstract: A sample-injection device has been developed at SPring-8 Angstrom Compact Free-Electron Laser (SACLA) for serial femtosecond crystallography (SFX) at atmospheric pressure. Microcrystals embedded in a highly viscous carrier are stably delivered from a capillary nozzle with the aid of a coaxial gas flow and a suction device. The cartridge-type sample reservoir is easily replaceable and facilitates sample reloading or exchange. The reservoir is positioned in a cooling jacket with a temperature-regulated water flow, which is useful to prevent drastic changes in the sample temperature during data collection. This work demonstrates that the injector successfully worked in SFX of the human A adenosine receptor complexed with an antagonist, ZM241385, in lipidic cubic phase and for hen egg-white lysozyme microcrystals in a grease carrier. The injection device has also been applied to many kinds of proteins, not only for static structural analyses but also for dynamics studies using pump-probe techniques.

PubMed: 31798359
DOI: 10.1107/S1600576719012846

Experimental method

X-RAY DIFFRACTION (2.25 Å)