6JWP

crystal structure of EGOC

6JWP の概要

• エントリーDOI: 10.2210/pdb6jwp/pdb
• 分子名称: GTP-binding protein GTR1, GTP-binding protein GTR2, Protein MEH1, ... (7 entities in total)
• 機能のキーワード: egoc, roadblock domain, gtr1, torc1, protein transport
• 由来する生物種: Saccharomyces cerevisiae S288c (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 233955.90

構造登録者

Zhang, T.,Ding, J. (登録日: 2019-04-21, 公開日: 2019-12-11, 最終更新日: 2023-11-22)

主引用文献

Zhang, T.,Peli-Gulli, M.P.,Zhang, Z.,Tang, X.,Ye, J.,De Virgilio, C.,Ding, J.
Structural insights into the EGO-TC-mediated membrane tethering of the TORC1-regulatory Rag GTPases.
Sci Adv, 5:eaax8164-eaax8164, 2019

PubMed Abstract: The Rag/Gtr GTPases serve as a central module in the nutrient-sensing signaling network upstream of TORC1. In yeast, the anchoring of Gtr1-Gtr2 to membranes depends on the Ego1-Ego2-Ego3 ternary complex (EGO-TC), resulting in an EGO-TC-Gtr1-Gtr2 complex (EGOC). EGO-TC and human Ragulator share no obvious sequence similarities and also differ in their composition with respect to the number of known subunits, which raises the question of how the EGO-TC fulfills its function in recruiting Gtr1-Gtr2. Here, we report the structure of EGOC, in which Ego1 wraps around Ego2, Ego3, and Gtr1-Gtr2. In addition, Ego3 interacts with Gtr1-Gtr2 to stabilize the complex. The functional roles of key residues involved in the assembly are validated by in vivo assays. Our structural and functional data combined demonstrate that EGOC and Ragulator-Rag complex are structurally conserved and that EGO-TC is essential and sufficient to recruit Gtr1-Gtr2 to membranes to ensure appropriate TORC1 signaling.

PubMed: 31579828
DOI: 10.1126/sciadv.aax8164

実験手法

X-RAY DIFFRACTION (3.2 Å)