6JW7

The crystal structure of KanD2 in complex with NADH and 3"-deamino-3"-hydroxykanamycin A

Summary for 6JW7

Entry DOI	10.2210/pdb6jw7/pdb
Descriptor	Dehydrogenase, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, (2R,3S,4S,5R,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-bis(azanyl)-3-[(2S,3R,4S,5S,6R)-6-(hydroxymethyl)-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-2-oxidanyl-cyclohexyl]oxy-oxane-3,4,5-triol, ... (4 entities in total)
Functional Keywords	rossmann fold, nad binding, oxidoreductase
Biological source	Streptomyces kanamyceticus
Total number of polymer chains	2
Total formula weight	84032.48
Authors	Kudo, F.,Kitayama, Y.,Miyanaga, A.,Hirayama, A.,Eguchi, T. (deposition date: 2019-04-18, release date: 2020-04-15, Last modification date: 2023-11-22)
Primary citation	Kudo, F.,Kitayama, Y.,Miyanaga, A.,Hirayama, A.,Eguchi, T. Biochemical and structural analysis of a dehydrogenase, KanD2, and an aminotransferase, KanS2, that are responsible for the construction of the kanosamine moiety in kanamycin biosynthesis. Biochemistry, 59:1470-1473, 2020 Cited by PubMed: 32237736 DOI: 10.1021/acs.biochem.0c00204 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.36 Å)

Structure validation