6JUK

Crystal structure of Formate dehydrogenase mutant C256I/E261P/S381I from Pseudomonas sp. 101 in complex with non-natural cofactor Nicotinamide Cytosine Dinucleotide

6JUK の概要

• エントリーDOI: 10.2210/pdb6juk/pdb
• 分子名称: Formate dehydrogenase, GLYCEROL, [[(2S,3S,4R,5S)-5-(3-aminocarbonylpyridin-1-ium-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2S,3S,4R,5S)-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl hydrogen phosphate, ... (4 entities in total)
• 機能のキーワード: formate dehydrogenase, oxidoreductase
• 由来する生物種: Pseudomonas sp. 101 (Achromobacter parvulus T1)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90229.75

構造登録者

Feng, Y.,Xue, S.,Guo, X.,Zhao, Z. (登録日: 2019-04-14, 公開日: 2020-05-13, 最終更新日: 2023-11-22)

主引用文献

Guo, X.,Wang, X.,Liu, Y.,Li, Q.,Wang, J.,Liu, W.,Zhao, Z.K.
Structure-Guided Design of Formate Dehydrogenase for Regeneration of a Non-Natural Redox Cofactor.
Chemistry, 26:16611-16615, 2020

PubMed Abstract: Formate dehydrogenase (FDH) has been widely used for the regeneration of the reduced nicotinamide adenine dinucleotide (NADH). To utilize nicotinamide cytosine dinucleotide (NCD) as a non-natural redox cofactor, it remains challenging as NCDH, the reduced form of NCD, has to be efficiently regenerated. Here we demonstrate successful engineering of FDH for NCDH regeneration. Guided by the structural information of FDH from Pseudomonas sp. 101 (pseFDH) and the NAD-pseFDH complex, semi-rational strategies were applied to design mutant libraries and screen for NCD-linked activity. The most active mutant reached a cofactor preference switch from NAD to NCD by 3700-fold. Homology modeling analysis showed that these mutants had reduced cofactor binding pockets and dedicated hydrophobic interactions for NCD. Efficient regeneration of NCDH was implemented by powering an NCD-dependent D-lactate dehydrogenase for stoichiometric and stereospecific reduction of pyruvate to D-lactate at the expense of formate.

PubMed: 32815230
DOI: 10.1002/chem.202003102

実験手法

X-RAY DIFFRACTION (2.293 Å)