6JUF

SspB crystal structure

6JUF の概要

• エントリーDOI: 10.2210/pdb6juf/pdb
• 分子名称: SspB protein (2 entities in total)
• 機能のキーワード: dnase, pt, hydrolase
• 由来する生物種: Streptomyces clavuligerus (strain ATCC 27064 / DSM 738 / JCM 4710 / NBRC 13307 / NCIMB 12785 / NRRL 3585 / VKM Ac-602)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 82496.80

構造登録者

Liqiong, L.,Yubing, Z. (登録日: 2019-04-13, 公開日: 2020-03-25, 最終更新日: 2024-03-27)

主引用文献

Xiong, X.,Wu, G.,Wei, Y.,Liu, L.,Zhang, Y.,Su, R.,Jiang, X.,Li, M.,Gao, H.,Tian, X.,Zhang, Y.,Hu, L.,Chen, S.,Tang, Y.,Jiang, S.,Huang, R.,Li, Z.,Wang, Y.,Deng, Z.,Wang, J.,Dedon, P.C.,Chen, S.,Wang, L.
SspABCD-SspE is a phosphorothioation-sensing bacterial defence system with broad anti-phage activities.
Nat Microbiol, 5:917-928, 2020

PubMed Abstract: Bacteria have evolved diverse mechanisms to fend off predation by bacteriophages. We previously identified the Dnd system, which uses DndABCDE to insert sulfur into the DNA backbone as a double-stranded phosphorothioate (PT) modification, and DndFGH, a restriction component. Here, we describe an unusual SspABCD-SspE PT system in Vibrio cyclitrophicus, Escherichia coli and Streptomyces yokosukanensis, which has distinct genetic organization, biochemical functions and phenotypic behaviour. SspABCD confers single-stranded and high-frequency PTs with SspB acting as a nickase and possibly introducing nicks to facilitate sulfur incorporation. Strikingly, SspABCD coupled with SspE provides protection against phages in unusual ways: (1) SspE senses sequence-specific PTs by virtue of its PT-stimulated NTPase activity to exert its anti-phage activity, and (2) SspE inhibits phage propagation by introducing nicking damage to impair phage DNA replication. These results not only expand our knowledge about the diversity and functions of DNA PT modification but also enhance our understanding of the known arsenal of defence systems.

PubMed: 32251370
DOI: 10.1038/s41564-020-0700-6

実験手法

X-RAY DIFFRACTION (1.587 Å)