6JO7

Crystal structure of mouse MXRA8

6JO7 の概要

• エントリーDOI: 10.2210/pdb6jo7/pdb
• 分子名称: Matrix remodeling-associated protein 8 (2 entities in total)
• 機能のキーワード: arthritogenic alphaviruses, receptor, virus entry, chikungunya virus, protein binding
• 由来する生物種: Mus musculus (Mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61448.76

構造登録者

Song, H.,Zhao, Z.,Qi, J.,Gao, F.,Gao, G.F. (登録日: 2019-03-20, 公開日: 2019-05-15, 最終更新日: 2024-10-16)

主引用文献

Song, H.,Zhao, Z.,Chai, Y.,Jin, X.,Li, C.,Yuan, F.,Liu, S.,Gao, Z.,Wang, H.,Song, J.,Vazquez, L.,Zhang, Y.,Tan, S.,Morel, C.M.,Yan, J.,Shi, Y.,Qi, J.,Gao, F.,Gao, G.F.
Molecular Basis of Arthritogenic Alphavirus Receptor MXRA8 Binding to Chikungunya Virus Envelope Protein.
Cell, 177:1714-1724.e12, 2019

PubMed Abstract: Arthritogenic alphaviruses, such as Chikungunya virus (CHIKV), cause severe and debilitating rheumatic diseases worldwide, resulting in severe morbidity and economic costs. Recently, MXRA8 was reported as an entry receptor. Here, we present the crystal structures of the mouse MXRA8, human MXRA8 in complex with the CHIKV E protein, and the cryo-electron microscopy structure of human MXRA8 and CHIKV virus-like particle. MXRA8 has two Ig-like domains with unique structural topologies. This receptor binds in the "canyon" between two protomers of the E spike on the surface of the virion. The atomic details at the interface between the two binding entities reveal that both the two domains and the hinge region of MXRA8 are involved in interaction with CHIKV E1-E2 residues from two protomers. Notably, the stalk region of MXRA8 is critical for CHIKV virus entry. This finding provides important information regarding the development of therapeutic countermeasures against those arthritogenic alphaviruses.

PubMed: 31080063
DOI: 10.1016/j.cell.2019.04.008

実験手法

X-RAY DIFFRACTION (2.4 Å)