6JLN

XFEL structure of cyanobacterial photosystem II (1F state, dataset2)

6JLN の概要

• エントリーDOI: 10.2210/pdb6jln/pdb
• 関連するPDBエントリー: 6JLM 6JLO 6JLP
• 分子名称: Photosystem II protein D1, Photosystem II reaction center protein K, Photosystem II reaction center protein L, ... (41 entities in total)
• 機能のキーワード: photosystem ii, photosynthesis, psii, membrane protein
• 由来する生物種: Thermosynechococcus vulcanus; 詳細
• タンパク質・核酸の鎖数: 39
• 化学式量合計: 730277.56

構造登録者

Suga, M.,Shen, J.R. (登録日: 2019-03-06, 公開日: 2019-10-16, 最終更新日: 2025-12-17)

主引用文献

Suga, M.,Akita, F.,Yamashita, K.,Nakajima, Y.,Ueno, G.,Li, H.,Yamane, T.,Hirata, K.,Umena, Y.,Yonekura, S.,Yu, L.J.,Murakami, H.,Nomura, T.,Kimura, T.,Kubo, M.,Baba, S.,Kumasaka, T.,Tono, K.,Yabashi, M.,Isobe, H.,Yamaguchi, K.,Yamamoto, M.,Ago, H.,Shen, J.R.
An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser.
Science, 366:334-338, 2019

PubMed Abstract: Photosynthetic water oxidation is catalyzed by the MnCaO cluster of photosystem II (PSII) with linear progression through five S-state intermediates (S to S). To reveal the mechanism of water oxidation, we analyzed structures of PSII in the S, S, and S states by x-ray free-electron laser serial crystallography. No insertion of water was found in S, but flipping of D1 Glu upon transition to S leads to the opening of a water channel and provides a space for incorporation of an additional oxygen ligand, resulting in an open cubane MnCaO cluster with an oxyl/oxo bridge. Structural changes of PSII between the different S states reveal cooperative action of substrate water access, proton release, and dioxygen formation in photosynthetic water oxidation.

PubMed: 31624207
DOI: 10.1126/science.aax6998

実験手法

X-RAY DIFFRACTION (2.4 Å)