6JLI

Crystal structure of CTLD7 domain of human PLA2R

6JLI の概要

• エントリーDOI: 10.2210/pdb6jli/pdb
• 分子名称: Secretory phospholipase A2 receptor, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: imn, epitope, pla2r, ctld7, immune system
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15540.28

構造登録者

Yu, B.,Hu, Z.,Kong, D.,Cheng, C.,He, Y. (登録日: 2019-03-06, 公開日: 2019-07-17, 最終更新日: 2024-10-16)

主引用文献

Yu, B.,Hu, Z.,Kong, D.,Cheng, C.,He, Y.
Crystal structure of the CTLD7 domain of human M-type phospholipase A2 receptor.
J.Struct.Biol., 207:295-300, 2019

PubMed Abstract: M-type phospholipase A2 receptor (PLA2R) is a member of the mannose receptor family. Recent evidence shows that PLA2R is a major autoantigen causing idiopathic membranous nephropathy (IMN), which is an autoimmune disease and one of the most common causes for nephrotic syndrome in adults. The epitope mapping data suggest that the major epitopes of PLA2R locate at the CysR, CTLD1 and CTLD7 domains. However, due to the lack of the high-resolution structural information, it is unclear how the autoantibodies interact with PLA2R. Here we determine the crystal structure of the CTLD7 domain of PLA2R at 1.8 Å, showing that it adopts a typical CTLD fold, and the structural alignments also provide hints for the potential antibody binding regions. In addition, the high-resolution structural information of CTLD7 could be applied to identify the epitopes for autoantibodies, which would facilitate the therapeutic strategies against IMN.

PubMed: 31271865
DOI: 10.1016/j.jsb.2019.06.007

実験手法

X-RAY DIFFRACTION (1.778 Å)