6JGV

Crystal structure of the transcriptional regulator CadR from P. putida

6JGV の概要

• エントリーDOI: 10.2210/pdb6jgv/pdb
• 分子名称: CadR (2 entities in total)
• 機能のキーワード: cadr, merr family, cadmium regulator, transcription
• 由来する生物種: Pseudomonas putida (Arthrobacter siderocapsulatus)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 33343.41

構造登録者

Liu, X.C.,Gan, J.H.,Chen, H. (登録日: 2019-02-15, 公開日: 2019-09-25, 最終更新日: 2023-11-22)

主引用文献

Liu, X.,Hu, Q.,Yang, J.,Huang, S.,Wei, T.,Chen, W.,He, Y.,Wang, D.,Liu, Z.,Wang, K.,Gan, J.,Chen, H.
Selective cadmium regulation mediated by a cooperative binding mechanism in CadR.
Proc.Natl.Acad.Sci.USA, 116:20398-20403, 2019

PubMed Abstract: Detoxification of the highly toxic cadmium element is essential for the survival of living organisms. CadR, a MerR family transcriptional regulator, has been reported to exhibit an ultraspecific response to the cadmium ion. Our crystallographic and spectroscopic studies reveal that the extra cadmium selectivity of CadR is mediated by the unexpected cooperation of thiolate-rich site I and histidine-rich site II. Cadmium binding in site I mediates the reorientation of protein domains and facilitates the assembly of site II. Subsequently, site II bridge-links 2 DNA binding domains through ligands His140/His145 in the C-terminal histidine-rich tail. With dynamic transit between 2 conformational states, this bridge could stabilize the regulator into an optimal conformation that is critical for enhancing the transcriptional activity of the cadmium detoxification system. Our results provide dynamic insight into how nature utilizes the unique cooperative binding mechanism in multisite proteins to recognize cadmium ions specifically.

PubMed: 31548408
DOI: 10.1073/pnas.1908610116

実験手法

X-RAY DIFFRACTION (2.2 Å)