6JDP

the imm52 family protein TsiT (PA3908) from Pseudomonas aeruginosa

Summary for 6JDP

• Entry DOI: 10.2210/pdb6jdp/pdb
• Descriptor: Imm52 family protein, PHOSPHATE ION, SODIUM ION, ... (4 entities in total)
• Functional Keywords: immunity protein, monomer, t6ss, antitoxin
• Biological source: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
• Total number of polymer chains: 1
• Total formula weight: 30077.76

Authors

She, Z. (deposition date: 2019-02-02, release date: 2019-03-20, Last modification date: 2024-03-27)

Primary citation

She, Z.,Geng, Z.,Xu, J.H.,Li, Y.H.,Dong, Y.H.
Structural characterization of the Imm52 family protein TsiT in Pseudomonas aeruginosa.
Protein Sci., 28:971-975, 2019

PubMed Abstract: The bacterial type VI secretion system (T6SS) utilizes many toxic effectors to gain advantage over interbacterial competition and eukaryotic host infection. Meanwhile, the cognate immunity proteins of these effectors are employed to protect themselves from the virulence. TseT and TsiT form an effector-immunity (E-I) protein pair secreted by T6SS of Pseudomonas aeruginosa. TseT is toxic for other bacteria, whereas TsiT can suppress the virulence of TseT. Here, we report the crystal structure of TsiT at 1.6 Å resolution. TsiT is a typical α + β class protein and belongs to a novel Imm52 protein family of the polymorphic toxin system. Apart from TsiT, only one structure of the Imm52 family proteins is present in the Protein Data Bank (PDB), but that structure is not characterized and shares low sequence identity with TsiT. We characterized the basic features of TsiT structure and identified conserved residues of the Imm52 family proteins according to homology comparison. Our work provided structural information of a new protein family and should aid future functional studies.

PubMed: 30834616
DOI: 10.1002/pro.3597

Experimental method

X-RAY DIFFRACTION (1.601 Å)