6JD9

Proteus mirabilis lipase mutant - I118V/E130G

6JD9 の概要

• エントリーDOI: 10.2210/pdb6jd9/pdb
• 分子名称: Alpha/beta hydrolase, CALCIUM ION, (4S)-2-METHYL-2,4-PENTANEDIOL, ... (4 entities in total)
• 機能のキーワード: lipase, proteus mirabilis lipase, hydrolase
• 由来する生物種: Proteus mirabilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31738.79

構造登録者

Heater, B.S.,Chan, W.S.,Chan, M.K. (登録日: 2019-01-31, 公開日: 2019-07-24, 最終更新日: 2024-03-27)

主引用文献

Heater, B.S.,Chan, W.S.,Lee, M.M.,Chan, M.K.
Directed evolution of a genetically encoded immobilized lipase for the efficient production of biodiesel from waste cooking oil.
Biotechnol Biofuels, 12:165-165, 2019

PubMed Abstract: We have recently developed a one-step, genetically encoded immobilization approach based on fusion of a target enzyme to the self-crystallizing protein Cry3Aa, followed by direct production and isolation of the fusion crystals from . Using this approach, lipase A was genetically fused to Cry3Aa to produce a Cry3Aa-lipA catalyst capable of the facile conversion of coconut oil into biodiesel over 10 reaction cycles. Here, we investigate the fusion of another lipase to Cry3Aa with the goal of producing a catalyst suitable for the conversion of waste cooking oil into biodiesel.

PubMed: 31297153
DOI: 10.1186/s13068-019-1509-5

実験手法

X-RAY DIFFRACTION (1.58 Å)