6JCE

NMR solution and X-ray crystal structures of a DNA containing both right-and left-handed parallel-stranded G-quadruplexes

6JCE の概要

• エントリーDOI: 10.2210/pdb6jce/pdb
• 分子名称: 29-mer DNA (1 entity in total)
• 機能のキーワード: left-handed, z-g4, g-quadruplex, hybrid, dna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9201.85

構造登録者

Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Mechulam, Y.,Schmitt, E.,Phan, A.T. (登録日: 2019-01-28, 公開日: 2019-07-10, 最終更新日: 2024-05-15)

主引用文献

Winnerdy, F.R.,Bakalar, B.,Maity, A.,Vandana, J.J.,Mechulam, Y.,Schmitt, E.,Phan, A.T.
NMR solution and X-ray crystal structures of a DNA molecule containing both right- and left-handed parallel-stranded G-quadruplexes.
Nucleic Acids Res., 47:8272-8281, 2019

PubMed Abstract: Analogous to the B- and Z-DNA structures in double-helix DNA, there exist both right- and left-handed quadruple-helix (G-quadruplex) DNA. Numerous conformations of right-handed and a few left-handed G-quadruplexes were previously observed, yet they were always identified separately. Here, we present the NMR solution and X-ray crystal structures of a right- and left-handed hybrid G-quadruplex. The structure reveals a stacking interaction between two G-quadruplex blocks with different helical orientations and displays features of both right- and left-handed G-quadruplexes. An analysis of loop mutations suggests that single-nucleotide loops are preferred or even required for the left-handed G-quadruplex formation. The discovery of a right- and left-handed hybrid G-quadruplex further expands the polymorphism of G-quadruplexes and is potentially useful in designing a left-to-right junction in G-quadruplex engineering.

PubMed: 31216034
DOI: 10.1093/nar/gkz349

実験手法

SOLUTION NMR