6JC4

Crystal structure of the urease accessory protein UreF from Klebsiella pneumoniae

6JC4 の概要

• エントリーDOI: 10.2210/pdb6jc4/pdb
• 分子名称: Urease accessory protein UreF (2 entities in total)
• 機能のキーワード: nickel-binding; urease accessory protein, metal binding protein
• 由来する生物種: Klebsiella pneumoniae
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 114848.05

構造登録者

Wu, W.,Zhang, Q.,Bartlam, M. (登録日: 2019-01-28, 公開日: 2020-01-29, 最終更新日: 2024-05-29)

主引用文献

Liu, S.,Wu, W.,Zhao, Q.,Liang, H.,Che, S.,Zhang, H.,Liu, R.,Zhang, Q.,Bartlam, M.
Structural characterization of the urease accessory protein UreF from Klebsiella pneumoniae.
Acta Crystallogr.,Sect.F, 78:75-80, 2022

PubMed Abstract: Klebsiella pneumoniae is an opportunistic pathogen that mostly affects those with weakened immune systems. Urease is a vital enzyme that can hydrolyze urea to ammonia and carbon dioxide as a source of nitrogen for growth. Urease is also a K. pneumoniae virulence factor that enables survival of the bacterium under nutrient-limiting conditions. UreF, an important nickel-binding urease accessory protein, is involved in the insertion of Ni into the active site of urease. Here, the crystal structure of UreF from K. pneumoniae (KpUreF) is reported. Functional data show that KpUreF forms a stable dimer in solution. These results may provide a starting point for the design of urease inhibitors.

PubMed: 35102896
DOI: 10.1107/S2053230X22000474

実験手法

X-RAY DIFFRACTION (2.3 Å)