6JC3

The Cryo-EM structure of nucleoprotein-RNA complex of Newcastle disease virus

6JC3 の概要

• エントリーDOI: 10.2210/pdb6jc3/pdb
• EMDBエントリー: 9793
• 分子名称: Nucleocapsid, polyU (2 entities in total)
• 機能のキーワード: self-capping helical structure complex, nuclear protein, nuclear protein-rna complex, nuclear protein/rna
• 由来する生物種: Avian avulavirus 1; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45622.74

構造登録者

Song, X.,Shan, H.,Zhu, Y.,Ding, W.,Ouyang, S.,Shen, Q.T.,Liu, Z.J. (登録日: 2019-01-28, 公開日: 2019-08-07, 最終更新日: 2024-03-27)

主引用文献

Song, X.,Shan, H.,Zhu, Y.,Hu, S.,Xue, L.,Chen, Y.,Ding, W.,Niu, T.,Gu, J.,Ouyang, S.,Shen, Q.T.,Liu, Z.J.
Self-capping of nucleoprotein filaments protects the Newcastle disease virus genome.
Elife, 8:-, 2019

PubMed Abstract: Non-segmented negative-strand RNA viruses, such as measles, ebola and Newcastle disease viruses (NDV), encapsidate viral genomic RNAs into helical nucleocapsids, which serve as the template for viral replication and transcription. Here, the clam-shaped nucleocapsid structure, where the NDV viral genome is sequestered, was determined at 4.8 Å resolution by cryo-electron microscopy. The clam-shaped structure is composed of two single-turn spirals packed in a back-to-back mode. This tightly packed structure functions as a seed for the assembly of a nucleocapsid from both directions, facilitating the growth of double-headed filaments with two separate RNA strings inside. Disruption of this structure by mutations in its loop interface yielded a single-headed unfunctional filament.

PubMed: 31290740
DOI: 10.7554/eLife.45057

実験手法

ELECTRON MICROSCOPY (4.8 Å)