6JBQ

CryoEM structure of Escherichia coli sigmaE transcription initiation complex containing 5nt of RNA

6JBQ の概要

• エントリーDOI: 10.2210/pdb6jbq/pdb
• EMDBエントリー: 9792
• 分子名称: DNA-directed RNA polymerase subunit alpha, ZINC ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
• 機能のキーワード: rna polymerase, extra-cytoplasmic function sigma factors, sigmae, transcription initiation, transcription-dna-rna complex, transcription/dna/rna
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 447160.35

構造登録者

Fang, C.L.,Zhang, Y. (登録日: 2019-01-26, 公開日: 2019-05-29, 最終更新日: 2024-03-27)

主引用文献

Fang, C.,Li, L.,Shen, L.,Shi, J.,Wang, S.,Feng, Y.,Zhang, Y.
Structures and mechanism of transcription initiation by bacterial ECF factors.
Nucleic Acids Res., 47:7094-7104, 2019

PubMed Abstract: Bacterial RNA polymerase (RNAP) forms distinct holoenzymes with extra-cytoplasmic function (ECF) σ factors to initiate specific gene expression programs. In this study, we report a cryo-EM structure at 4.0 Å of Escherichia coli transcription initiation complex comprising σE-the most-studied bacterial ECF σ factor (Ec σE-RPo), and a crystal structure at 3.1 Å of Mycobacterium tuberculosis transcription initiation complex with a chimeric σH/E (Mtb σH/E-RPo). The structure of Ec σE-RPo reveals key interactions essential for assembly of E. coli σE-RNAP holoenzyme and for promoter recognition and unwinding by E. coli σE. Moreover, both structures show that the non-conserved linkers (σ2/σ4 linker) of the two ECF σ factors are inserted into the active-center cleft and exit through the RNA-exit channel. We performed secondary-structure prediction of 27,670 ECF σ factors and find that their non-conserved linkers probably reach into and exit from RNAP active-center cleft in a similar manner. Further biochemical results suggest that such σ2/σ4 linker plays an important role in RPo formation, abortive production and promoter escape during ECF σ factors-mediated transcription initiation.

PubMed: 31131408
DOI: 10.1093/nar/gkz470

実験手法

ELECTRON MICROSCOPY (4.02 Å)