6J9A

Crystal structure of Arabidopsis thaliana VAL1 in complex with FLC DNA fragment

6J9A の概要

• エントリーDOI: 10.2210/pdb6j9a/pdb
• 分子名称: B3 domain-containing transcription repressor VAL1, DNA (5'-D(*AP*TP*TP*CP*TP*GP*CP*AP*TP*GP*GP*AP*TP*TP*G)-3'), DNA (5'-D(*AP*AP*TP*CP*CP*AP*TP*GP*CP*AP*GP*AP*AP*TP*C)-3') (3 entities in total)
• 機能のキーワード: flowering time regulation, dna-binding, gene regulation, transcription, transcription-dna complex, transcription/dna
• 由来する生物種: Arabidopsis thaliana (Mouse-ear cress); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 24009.16

構造登録者

Hu, H.,Du, J. (登録日: 2019-01-22, 公開日: 2019-05-29, 最終更新日: 2024-10-23)

主引用文献

Tao, Z.,Hu, H.,Luo, X.,Jia, B.,Du, J.,He, Y.
Embryonic resetting of the parental vernalized state by two B3 domain transcription factors in Arabidopsis.
Nat.Plants, 5:424-435, 2019

PubMed Abstract: Some overwintering plants acquire competence to flower, after experiencing prolonged cold in winter, through a process termed vernalization. In the crucifer plant Arabidopsis thaliana, prolonged cold induces chromatin-mediated silencing of the potent floral repressor FLOWERING LOCUS C (FLC) by Polycomb proteins. This vernalized state is epigenetically maintained or 'memorized' in warm rendering plants competent to flower in spring, but is reset in the next generation. Here, we show that in early embryogenesis, two homologous B3 domain transcription factors LEAFY COTYLEDON 2 (LEC2) and FUSCA3 (FUS3) compete against two repressive B3-containing epigenome readers and Polycomb partners known as VAL1 and VAL2 for the cis-regulatory cold memory element (CME) of FLC to disrupt Polycomb silencing. Consistently, crystal structures of B3-CME complexes show that B3, B3 and B3 employ a nearly identical binding interface for CME. We further found that LEC2 and FUS3 recruit the scaffold protein FRIGIDA in association with active chromatin modifiers to establish an active chromatin state at FLC, which results in resetting of the silenced FLC to active and erasing the epigenetic parental memory of winter cold in early embryos. Following embryo development, LEC2 and FUS3 are developmentally silenced throughout post-embryonic stages, enabling VALs to bind to the CME again at seedling stages at which plants experience winter cold. Our findings illustrate how overwintering crucifer annuals or biennials in temperate climates employ a subfamily of B3 domain proteins to switch on, off and on again the expression of a key flowering gene in the embryo-to-plant-to-embryo cycle, and thus to synchronize growth and development with seasonal temperature changes in their life cycles.

PubMed: 30962525
DOI: 10.1038/s41477-019-0402-3

実験手法

X-RAY DIFFRACTION (2.915 Å)