6J8L

Phytophthora sojae effector PsAvh240 inhibits a host aspartic protease secretion to promote infection

6J8L の概要

• エントリーDOI: 10.2210/pdb6j8l/pdb
• 分子名称: Avh240, SULFATE ION (3 entities in total)
• 機能のキーワード: apoplastic immunity, effector, aspartic protease, immune system
• 由来する生物種: Phytophthora sojae (Soybean stem and root rot agent)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32589.17

構造登録者

Guo, B. (登録日: 2019-01-19, 公開日: 2019-02-06, 最終更新日: 2024-03-27)

主引用文献

Guo, B.,Wang, H.,Yang, B.,Jiang, W.,Jing, M.,Li, H.,Xia, Y.,Xu, Y.,Hu, Q.,Wang, F.,Yu, F.,Wang, Y.,Ye, W.,Dong, S.,Xing, W.,Wang, Y.
Phytophthora sojae Effector PsAvh240 Inhibits Host Aspartic Protease Secretion to Promote Infection.
Mol Plant, 12:552-564, 2019

PubMed Abstract: Plants secrete defense molecules into the extracellular space (the apoplast) to combat attacking microbes. However, the mechanisms by which successful pathogens subvert plant apoplastic immunity remain poorly understood. In this study, we show that PsAvh240, a membrane-localized effector of the soybean pathogen Phytophthora sojae, promotes P. sojae infection in soybean hairy roots. We found that PsAvh240 interacts with the soybean-resistant aspartic protease GmAP1 in planta and suppresses the secretion of GmAP1 into the apoplast. By solving its crystal structure we revealed that PsAvh240 contain six α helices and two WY motifs. The first two α helices of PsAvh240 are responsible for its plasma membrane-localization and are required for PsAvh240's interaction with GmAP1. The second WY motifs of two PsAvh240 molecules form a handshake arrangement resulting in a handshake-like dimer. This dimerization is required for the effector's repression of GmAP1 secretion. Taken together, these data reveal that PsAvh240 localizes at the plasma membrane to interfere with GmAP1 secretion, which represents an effective mechanism by which effector proteins suppress plant apoplastic immunity.

PubMed: 30703565
DOI: 10.1016/j.molp.2019.01.017

実験手法

X-RAY DIFFRACTION (2.3 Å)